Gene Expression in Individual Bovine Somatic Cell Cloned Embryos at the 8-cell and Blastocyst Stages of Preimplantation Development
نویسندگان
چکیده
منابع مشابه
Gene expression in individual bovine somatic cell cloned embryos at the 8-cell and blastocyst stages of preimplantation development.
Aberrant gene expression in somatic cell nuclear-transferred (NT) embryos due to abnormal epigenetic modifications of the donor nucleus likely accounts for much of the observed diminished viability and developmental abnormalities. We compared the expression of 13 developmentally important genes in individual 8-cell and blastocyst stage NT embryos produced from adults female cumulus cells and ad...
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background: the values of embryonic stem cell and cloning are evident. production of clone from embryonic stem cells can be achieved by introduction of stem cell into a tetraploid blastocyst. tetraploid blastocyst can be produced in vitro by electrofusion of 2-cell embryos. objective: the aim of this study was to assess the effect of different voltages and durations on fusion rate of bovine 2-c...
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We investigated the effects of exposure time and concentration of trichostatin A (TSA) on in vitro development and quality of bovine SCNT embryos. At multiple time points, the relative expression of genes related to pluripotency and reprogramming was analyzed in order to assess the quality of preimplantation embryos cultured in media with TSA using real-time PCR. Development into blastocysts wa...
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Background: Development of cloned bovine embryos. Materials and Methods: Oocytes collection,oocyte denudation, oocyte enucleation, nuclear transfer, electrofusion, reconstructed embryo activation, culture of reconstructed and IVF embryo,and treatment with SAH post fusion interval Treatment of reconstructed embryos with TSA for 12 hours after activation, preparation of somatic donor cells, donor...
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ژورنال
عنوان ژورنال: Journal of Reproduction and Development
سال: 2007
ISSN: 0916-8818,1348-4400
DOI: 10.1262/jrd.19096